Development and ELISA-based detection of anti-M2e IgY antibodies using an encoding plasmid for M2e-Hsp70 c-terminal gene

The use of IgYs in a variety of methods in different areas of research, diagnostics, medical application and biotechnology should be considered widely. Development of antibodies against extra cellular domain of influenza M2 [M2e] protein in egg yolk of laying hens. A Fusion construct harboring C-terminal of bovine heat shock protein 70 [Hsp70] and influenza M2e coding genes was injected to laying hens. Serum and egg yolk antibodies were screened for the presence of anti-M2e antibodies by indirect enzyme-linked immunosorbent assay [ELISA]. Anti-M2e antibodies were detected in egg yolks and sera of injected hens from 13 and 7 days post injection [PI], with the peak titer detected on 41 and 35 days PI, respectively. Anti-M2e IgY titers could be an index for expression potential of pcDNA3.1-M2e-HspCterminal construct in laying hens. This construct could be considered as a promising tool in production of anti-M2e polyclonal, monospecific IgY antibodies. Such anti-M2e antibodies could be exploited for influenza diagnostic and therapeutic measures

Qualitative and quantitative assessment of poultry carcasses contaminated with salmonella in Tehran industrial slaughter houses

Broiler carcasses are considered to be the major source of salmonella detection and enumeration of salmonella in this product are two criteria for evaluation of hygienic quality of the slaughterhouses and the product itself. In this study, contamination of broiler carcasses produced in poultry Industrial slaughterhouses in Tehran province was assessed using three tube MPN methods. According to our results 69 percent of the samples were salmonella positive while not detected the salmonella in the rest of samples. Enumeration of salmonella per carcass indicated that the average number of salmonella in 62/72 of salmonella positive carcasses was 49/5 and in 7/6 percent 2605 and in 16/48 percent 279/5 and in 2/3 percent upper 3300. Our results also showed that number of salmonella per per gram and 2 cm2 of positive carcasses in 93/42 percent were 0/054 MPN /gr and 0/047 MPN/cm and in 4/38 2 2 percent 1/695 MPN/gr and 1/4 MPN/cm and in 1/5 percent upper 1/7 MPN/gr and 1/5 MPN /cm. The statistical analysis in this study results and calculates the ratio and average indicates that the mean contamination of carcasses exanimate was low although frequency contamination was high